Screening of indigenous plants for acetylcholinesterase inhibition by TLC bioautographic method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
Acetylcholinesterase inhibition is considered as the most suitable therapeutic strategy for Alzheimer’s disease. As synthetic drugs lack selective activity, efficacy and bioavailability, many traditional remedies have been explored for improving cognitive function. In the present study, indigenous plants namely Mandookaparni, Liquorice, Gaduchi, Long pepper and Ukshi were screened for acetylcholinesterase inhibition. Methanolic extracts of selected plants were prepared by soxhlet extraction and subjected to preliminary solventsolvent extraction using dichloromethane and ethyl acetate. All the fractions were screened for acetylcholinesterase inhibition by a modified thin layer chromatography bioautographic method. Ethyl acetate fractions of Mandookaparni and Liquorice exhibited inhibition at Rf 0.74 and 0.76, respectively. Aqueous methanol fraction of Gaduchi showed significant inhibition at Rf 0.61 and 0.8 while this was at Rf 0.68 for Long pepper. The presence of active constituents like flavonoids, saponins and alkaloids seem to be responsible for the inhibitory activity observed in the present study. The selected indigenous plants could be important in the management of AD and identification of active constituents and further evaluation of their clinical efficacy and potential toxicity in larger trials is very much required.
Authors and Affiliations
E. N. Gaviraj, B. Shiva kumar, S. M. Biradar, B. S. Hunasagi, V. P. Patil, S. R. Karajgi
Keywords
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- EP ID EP415685
- DOI 10.9790/3008-1305052125.
- Views 80
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