Telomerase Positive Totipotent Stem Cells and Pluripotent Stem Cells in the Adult Brain I. Cerebral Cortex
Journal Title: Journal of Regenerative Medicine & Biology Research - Year 2021, Vol 2, Issue 1
Abstract
Previous isolation studies noted the presence of native undifferentiated telomerase positive stem cells within the brains of adult rats. Further segregation of the cells from the brain isolates demonstrated the presence of telomerase positive totipotent stem cells and pluripotent stem cells within the cell isolates. Characterization studies of their differentiation potential noted that both the telomerase positive totipotent stem cells and pluripotent stem cells would form neurons, ganglion cells and glial cells, as well as cells from surface ectoderm, mesodermal and endodermal embryonic germ layer lineages. Since reports from other groups noted the presence of neural stem cells within the subventricular zone of the lateral ventricles and the dentate gyrus of the hippocampus, we wanted to ascertain the locations of these native telomerase positive totipotent stem cells and pluripotent stem cells within various regions of the adult brain. The brains from adult rats were examined. Adult rats were euthanized following the guidelines of Mercer University School of Medicine’s IACUC. The rats were perfused with ELICA fixative, the brains harvested, frozen, cryosectioned and stained with antibodies diagnostic for the endogenous totipotent stem cells, i.e., carcinoembryonic antigen-cell adhesion molecule-1 (CEA-CAM-1) and pluripotent stem cells, i.e., stage-specific embryonic antigen-4 (SSEA-4). Both CEA-CAM-1 and SSEA-4 positive stem cells were located within the gray matter of the cerebral cortex, whereas SSEA-4 positive cells were also located in the white matter. These results suggest that the totipotent stem cells and pluripotent stem cells are a resident population of stem cells within the cerebral cortex of the adult brain. Studies are ongoing to address their functional significance in the brain.
Authors and Affiliations
Henry E Young*, Frank Lochner
Keywords
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- EP ID EP698852
- DOI http://dx.doi.org/10.46889/JRMBR.2021.2102
- Views 82
- Downloads 0
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